Theanine: What is it, Properties and Where to Find it

Theanine: What is it, Properties and Where to Find it

L-Theanine and Green Tea: Properties, Effects, and Contraindications

Initially identified as the flavor responsible for the sweet taste of some green teas, theanine has been the subject of scientific studies in recent decades that have revealed its potential relaxing, anxiolytic, and hypotensive activity. An extremely rare molecule to find in nature, theanine is responsible for the relaxed and serene wakefulness state we find ourselves in after drinking a cup of good green tea, such as matcha tea. But what are the mechanisms we can attribute to its properties? In this article, we will discover what L-theanine is, why it is so beneficial, and in which teas it is found.

theanine in tea

 

L-Theanine: What is it?

The L-theanine is an amino acid present in nature almost exclusively in tea, particularly in Camellia sinensis, the plant from whose leaves the famous infusion is obtained, especially in green tea and matcha tea. This important molecule, often associated with glutamine (in fact, its name is N-ethyl-L-glutamine) and derived from glutamic acid, as their chemical structure is very similar, differs from other amino acids in not being proteinogenic, meaning it is not incorporated into the chains that make up proteins.

Amino acids are molecules containing an amino group, a carboxylic group, and a chain that varies for different amino acids. Tea contains several amino acids, but only a portion of them are water-soluble and are retained in the infusion: theanine makes up 60% of this quantity of amino acids and is responsible for the recognizable sweet and umami taste of green tea, in which it is more abundant.

Theanine is a very important molecule, studied recently (especially since the 1950s) for its psychoactive properties, as it is able to cross the blood-brain barrier and induce alpha waves (or alpha rhythm) in the brain, usually recorded when the subject is awake but completely relaxed.

In this article, we want to delve into the extraordinary relaxing properties of theanine, as well as its other various health effects, discovering in which teas in particular it can be found and what benefits it has for our body.

theanine matcha tea

Where is Theanine Found?

The main source of theanine is tea, especially green tea, although traces have also been found in other species of the genus Camellia and in some varieties of mushrooms (Boletus badius). Tea represents the most common food in which it is possible to find L-theanine, which is therefore a substance as beneficial as it is difficult to find in nature.

Theanine is an amino acid that develops in the tea plant when the leaves are very young. As the plant undergoes chlorophyll photosynthesis thanks to exposure to the sun, the L-theanine transforms into catechins, the antioxidants of tea. Chemically, in the tea plant, there will be a greater amount of theanine in the younger and lighter leaves found at the top of the plant compared to the older lower leaves that have been exposed to the sun longer and have undergone photosynthesis and developed more chlorophyll. This does not mean that the first tea leaves are completely devoid of antioxidants: as the leaf remains exposed to the sun, however, it will lose theanine and gain antioxidants.

Not many people know that theanine affects the taste of tea: the more theanine is present in the leaf, the sweeter the tea will be. For this reason, a good matcha is recognizable by its characteristic sweet and velvety taste, due to the high levels of theanine. Few people know that theanine dissolves better in cold water: for this reason, you can opt for cold green tea to enjoy its benefits.

In pharmacies, L-theanine produced for commercialization is obtained through bacterial fermentation.

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theanine tea kabuse

Theanine in Matcha Tea and Green Tea

Among the green teas richest in theanine, we find matcha tea and gyokuro tea, known for their sweetness and balance. But why do these two teas have more theanine than other green teas?

The cultivation methods, the timing of leaf picking, and the climate are all factors that influence the chemical composition of the leaves, and therefore also the levels of theanine.

Matcha and gyokuro tea (like Gyokuro available at Terza Luna) have high levels of this amino acid mainly due to the traditional cultivation method in Japan called kabuse: with this cultivation, tea plants are shaded under tents, about 15 days before harvesting. Shading protects the plant from the sun: the leaves will produce less chlorophyll but will still mature. This way, a tea leaf rich in L-theanine is obtained, as well as caffeine and antioxidants.

Similarly, first flush leaves harvested in early spring, the so-called first flush, of any green tea (for example, sencha tea) will be richer in theanine compared to second flush leaves, harvested later, which have undergone more photosynthesis. Youthful tea leaves are consequently, thanks to their higher theanine content, more delicate, sweet, balanced, and therefore more precious, and will have fewer polyphenols, a characteristic of bitter taste.

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Properties of L-Theanine

But what is theanine for and why is it considered an indicator of tea quality? As already outlined, theanine has numerous properties: in particular, it is an amino acid that crosses the blood-brain barrier and increases alpha rhythm, the basal rhythm of electroencephalography in which you are awake but in a completely relaxed state. Brain waves are recorded through electroencephalogram that captures electrical impulses on the scalp: the rhythm and the different frequency and amplitude of the waves correspond to different brain activity, from wakefulness, to relaxation, to sleep. Alpha waves, or Berger's rhythm, are characterized by slow oscillations with a frequency of 8-13 Hz and indicate an awake, alert but relaxed adult and free from thoughts. Generally, this condition is nullified by visual or mental stimuli.

Experimental studies have shown that the administration of theanine in subjects with anxiety issues generated alpha brain waves 30 minutes after intake, corresponding to the absorption times of L-theanine and reaching brain tissue.

Several studies have also shown how the intake of theanine helps reduce physical and mental stress, inducing a feeling of relaxation but not drowsiness, thus improving attention and concentration. Thanks to the combined effect with caffeine present in tea leaves, theanine helps improve concentration and cognitive functions by counteracting the negative and anxiolytic effects of caffeine.

In general, based on the evidence found in scientific literature, the following properties are attributed to L-theanine:

  • Relaxing and Sedative
  • Anxiolytic and Hypotensive
  • Antidepressant
  • Neuroprotective
  • Anti-stress and fatigue
  • Immunomodulatory

gyokuro theanine

Relaxing and Anxiolytic Properties

Generally, alpha waves are recorded in that intermediate twilight where one is relaxed but not asleep, a state very favorable to the body and brain, which can also be achieved through meditation and breathing practices. It is the state experienced just before falling asleep, a state of extreme calm: conversely, a low level of alpha waves translates into anxiety, stress, and insomnia. Studies have shown how theanine stimulates the production of cerebral alpha waves, and therefore induces a state of relaxation and calm capable of counteracting anxiety, agitation, stress, and hypertension.

The exact mechanisms of theanine's reaction with serotonin levels are not known, but the effect produced by theanine in the brain is attributed to the increase in GABA production (gamma-aminobutyric acid), an important neurotransmitter that regulates the neuronal excitability of the nervous system, and dopamine levels in the brain. These mechanisms are precisely attributed to the relaxing, antidepressant, and anti-stress properties.

Some researchers believe that L-theanine regulates the excitotoxicity of glutamic acid—a precursor of theanine itself, as well as the neurotransmitter GABA—which high neuronal levels seem to favor headaches, insomnia, and excitement in addition to potentially causing neuronal damage and Alzheimer's disease. Studies conducted on laboratory rats have also shown neuroprotective effects of L-theanine.

Effects on the Immune System

L-theanine is also associated with immunomodulatory effects. It is believed that theanine improves the immune defenses' response to viruses, bacteria, and other infections by enhancing the activity of gamma delta T lymphocytes, which mediate innate immunity and play a role in antigen recognition. This action, however, requires further studies, as existing ones, while showing a correlation between theanine and immune response, have been conducted on limited samples.

matcha theanine

Theanine and Caffeine

Often, theanine is confused with caffeine, as its name is similar to that of theine, as caffeine is called in tea. In reality, they are two distinct molecules with different functions and effects, whose correlation is very important.

We have seen how the relaxing effect given by tea despite the caffeine content is attributable precisely to L-theanine, which counteracts the stimulating action of the central nervous system of theine, allowing for a state of calm but not drowsiness, a relaxed wakefulness in which the side effects associated with caffeine are modulated such as anxiety, tremors, nervousness, hyperexcitability, and tachycardia.

Often it is believed that at the leaf chemistry level, a tea containing more L-theanine will have less caffeine, but this belief is incorrect: the balance between the two occurs only in terms of the effects they have on the body. A matcha tea, or a Gyokuro, are teas rich in caffeine, which allow you to enjoy the energy and attention boost that this molecule induces, but at the same time rich in theanine, which thanks to its properties counteracts the negative effects associated with caffeine consumption, ensuring a state of alertness but also of psychophysical calm. Discover the matcha tea for sale online at Terza Luna ### How to Take Theanine? Tea and Supplements As we have already described, theanine is a substance difficult to find in nature, as it is found exclusively in tea, therefore in the leaves of Camellia sinensis, and in some mushrooms in limited quantities. Having discovered its benefits, the pharmaceutical industry produces dietary supplements with L-theanine for mood and sleep tone improvement and cognitive effects: in these cases, L-theanine is replicated by bacterial fermentation. Drinking tea remains the simplest way to take theanine, especially present in green tea and even more specifically in shaded teas such as matcha tea, gyokuro, and kabusecha. ![matcha theanine](https://www.terzaluna.com/image/catalog/blog/teanina/teanina-nel-matcha.jpg) ### How Much Theanine to Take? Dosage As we will see, there are no particular contraindications related to the intake of theanine, therefore if it is taken through tea, one must refer to the consequences of drinking too much tea. Although rich in theanine, a single cup of green tea will still provide a limited amount of this substance. The recommended doses vary between 100 and 200 mg. ### Contraindications Some studies show how theanine helps reduce blood pressure, which could increase the effect of antihypertensive drugs. Also, having relaxing properties, it could counteract the effect of drugs for improving cognitive activity. Theanine cannot be taken through green tea by people intolerant to caffeine, as the latter is still present in the infusion, although the effects are modulated by the substance. It is also preferable to avoid theanine in case of pregnancy and breastfeeding. ### Side Effects The intake of theanine is considered safe and too high doses of theanine do not have particular physical and psychological side effects. On the contrary, theanine has important effects on relaxation, anxiety, and insomnia, counteracting anxiety, stress, and improving concentration. Drinking a theanine-rich tea is optimal for those who suffer from the effects of caffeine but need a drink that wakes them up and helps maintain high attention, as the amino acid counteracts the negative effects and the anxiety induced by the theine, also countering the classic bitter taste of green tea due to caffeine.

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